DNA-RNA Amplification RT-PCR
Thermo Reverse Transcriptase
Improved efficiency in reverse transcription to synthesize full-length cDNA.
- First-strand cDNA synthesis
- Active at 42~55 ℃. Optimum at 50 ℃
- RNaseH negative
- Superior performance in RT-PCR
- High processivity (up to 12 kb)
Products
| Cat.No. | Product | Size |
|---|---|---|
| RT10K | HelixCript™ Thermo Reverse Transcriptase | 10,000 units |
| RT50K | HelixCript™ Thermo Reverse Transcriptase | 50,000 units |
| RT10KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP) | 10,000 units |
| RT50KN | HelixCript™ Thermo Reverse Transcriptase (with dNTP) | 50,000 units |
HelixCript™ Thermo Reverse Transcriptase, a thermostable and RNase H-negative variant of M-MLV RTase,
can synthesize cDNAs from purified RNA templates at a temperature range of 42 ℃ ~ 55 ℃ and especially
shows the highest activity at 50 ℃. At the escalated temperature, the cDNA synthesis is enhanced partially due
to the less internal structural formation of the template RNA and increased polymerization activity of RTase.
The high processivity and productivity of HelixCript™ Thermo Reverse Transcriptase allows this enzyme to
amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from RNA template.
This enzyme is quite suitable to synthesize the first-strand cDNA for RT-PCR of target gene. The cDNA synthesis
from total RNA or poly-(A) RNA is performed by random primer, oligo-d(T) primer, or gene-specific primer.
