DNA-RNA Amplification Direct Amplification
DirectFast qRT-PCR Kit
No RNA extraction. Speedy and simultaneous detection suitable for POC MDx.
- Direct: RNA extraction-free
- Fast(< 45 minutes) or ultrafast(< 25 minutes) for 40 cycles
- Multiplex: Up to 5-plex probes in a reaction
- Ab-based hot start Taq polymerase / Thermostable RTase / UDG system
Products
| Cat.No. | Product | Size |
|---|---|---|
| DFQRU-A100 | RealHelix™ DirectFast qRT-PCR Kit (V1a) | 100 rxns |
RealHelix™ DirectFast qRT-PCR Kit, a probe-based qRT-PCR kit, is designed for RNA direct real-time amplification from animal tissues, plant tissues, and various clinical samples (including whole blood, serum and swab collections) without any RNA purification processes. This kit allows the fast reaction to complete the qRT-PCR cycles within one hour. The enzyme mix in this kit is an optimized blend of reverse transcriptase, antibody-coupled Taq DNA polymerase, RNase inhibitor, and a heat-labile uracil-DNA-glycosylase (HL-UDG). The reaction buffer contains all of the required components, including optimized buffer components, Mg2+, dUTP, and dNTPs. The applied HL-UDG/dUTP system eliminates the carryover contamination of PCR products from previous reactions. HL-UDG efficiently removes uracil residues from dU-containing DNA during the PCR mixture setup and handling.
