HelixZyme™ Cod UDG is a recombinant Uracil DNA glycosylase from Gadus morhua expressed in E. coli. UDG catalyzes the hydrolysis of the N glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil containing single or double stranded DNA. Cod UDG is fully active in the temperature range of 15 to 25℃ and inactivated at over 50℃. Due to this character, this enzyme is ideal for applying on the one step RT PCR system to remove the contaminated uracil containing DNA in the reaction.

Concentration

• Cod UDG : 1 unit/μl

Storage

• Below -20 ℃

Shelf life

• 12 months

Storage buffer

• 20 mM Tris-HCl (pH 8.0)
• 50 mM NaCl
• 1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol