Detection Kit
Pathogenic Amoeba Detection Kit
- Simultaneous detection of N. fowleri and Acanthamoeba spp in one tube reaction
- Probe-based real-time PCR kit
- High specificity: Chemically-modified hot start Taq application
Products
| Cat.No. | Product | Size |
|---|---|---|
| AMQP50 | Pathogenic Amoeba Detection Kit | 50 rxns |
Naegleria fowleri(commonly referred to as the "brain-eating amoeba" or "brain-eating ameba"), is a free-living microscopic ameba(single-celled living organism). It can cause a rare and devastating infection of the brain called primary amebic meningoencephalitis(PAM). Naegleria fowleri usually infects people when contaminated water enters the body through the nose. Once the ameba enters the nose, it travels to the brain where it causes PAM, which is usually fatal.
Acanthamoeba is a microscopic, free-living ameba, or amoeba(single-celled living organism), that can cause rare, but severe infections of the eye, skin, and central nervous system. Acanthamoeba can be spread to the eyes through contact lens use, cuts, or skin wounds or by being inhaled into the lungs.
RealHelix™ Pathogenic Amoeba Detection Kit is a TaqMan® probe-based real-time PCR assay for the simultaneous detection of Naegleria fowleri and Acanthamoeba spp. in clinical samples. 2x qPCR premix contains hot-start PCR enzyme, dNTPs, buffers, Mg2+, and stabilizing agent. The hot-start PCR enzymes provide highly specific amplification of target DNA and minimize side products such as primer dimers. Based on the TaqMan® probe detection principle, the 5’-reporter dye and 3’-quencher dual-labeled oligonucleotide(TaqMan® probe) hybridize on a specific region within the amplified fragment. Target pathogen amplification is detected using FAM and HEX channel.
