This is the bulk version of HelixZyme™ Heat Labile UDG.

If you would like to purchase in bulk, please contact sale@nanohelix.net.

HelixZyme™ Heat Labile UDG is a recombinant uracil DNA glycosylase from marine bacterium BMTU 3346. UDG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. Heat Labile UDG is fully active in the temperature range of 15 to 25 ℃ and inactivated at over 50 ℃. Due to this characteristic, this enzyme is ideal for applying to a one-step RT-PCR system to remove the contaminated uracil-containing DNA in the reaction.

Application

• Prevention of carryover contamination in RT-PCR

Enzyme activities: Hydrolyzes uracil-glycosidic bonds in single- and double-stranded DNA.

Heat inactivation: +50 ℃ for 2 minutes are sufficient for inactivation.

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

Purity: ≥ 90 % (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 10 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

Activity test: Removal of uracil-containing PCR product with 1 unit of enzyme

Stability: 12 months at -20 ℃