This is the bulk version of HelixAmp™ Premium-Taq Polymerase.

If you would like to purchase in bulk, please contact sale@nanohelix.net.

​HelixAmp™ Premium-Taq Polymerase provides the most specific amplification using a dual system of antibody-mediated hot start polymerase and PMT (Polymerase Modulator on Temperature)-applied buffer. Anti-Taq antibody inhibits the Taq polymerase activity until it reaches denaturation temperature while the PMT-applied buffer reduces the primer-dimer formation and non-specific amplification during the PCR cycles. The separately available 5x reaction buffer contains pH-buffering agents, salts, Mg²⁺, dNTPs and PMT additive.

Application

• Antibody-mediated hot-start PCR
• Highly specific amplification while minimizing primer dimer formation
• Fast PCR assays with a short enzyme activation time and fast cycling
• Multiplex PCR
• PCR for molecular diagnostics

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Enzyme activation: 2 min at +95 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 12 months at -20 ℃