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HelixAmp™ Ab+Taq Polymerase is an anti-Taq antibody complex form of Taq polymerase and ideal for automatic hot-start PCR. This antibody-mediated hot-start polymerase provides highly specific and sensitive PCR amplification. The separately available 10x reaction buffer contains pH-buffering agents, salts, Mg²⁺, and dNTPs.

Application

• Antibody-mediated Hot-start PCR
• Highly specific conventional and real-time PCR
• Fast PCR assays with a short enzyme activation time and fast cycling protocols
• Multiplex PCR
• PCR for molecular diagnostics

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Enzyme activation: 2 min at +95 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)

Stability: 24 months at -20 ℃