This is the bulk version of HelixAmp™ Taq Polymerase.

If you would like to purchase in bulk, please contact sale@nanohelix.net.

HelixAmp™ Taq Polymerase, Glycerol-free is a lyophilization-compatible version of a highly purified recombinant standard PCR enzyme. Host DNAs are removed almost entirely from the enzyme to minimize false positive reactions during molecular diagnostic applications. The separately available 10x reaction buffer contains pH-buffering agents, salts, Mg²⁺, and dNTPs.

Application

• Routine PCR and RT-PCR
• Conventional and Real-Time PCR
• PCR for molecular diagnostics
• Manufacture of amplification mixtures

Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity

Temperature optimum: Approximately +75 ℃

Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and stabilizers

Purity: ≥ 95 % (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)

RNase contamination test: Not detectable (Incubation with 40 U enzyme and human total RNA at 37 ℃, 1 hr)

DNA contamination test: [E.coli DNA] less than 1 copy/5 U enzyme, [Human DNA] Not detectable

Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)