DNA-RNA Amplification Real-Time PCR & RT-PCR Real-Time RT-PCR
qRT-PCR Kit [Probe]
![qRT-PCR_Kit_[Probe]](/upload_files/editor/202411/QRT2-P200.jpg "QRT2-P200")
Easy, one-tube RT-PCR analysis with superior performances with probe set.
- Sensitive and reliable one-step quantitative RT-PCR kit
- Detection by sequence-specific probe
- High specificity using Hot-Taq polymerase
- Separately supplied ROX passive reference dye
Products
| Cat.No. | Product | Size |
|---|---|---|
| QRT2-P200 | RealHelix™ qRT-PCR Kit [Probe] | 200 rxns |
| QRT2-P500 | RealHelix™ qRT-PCR Kit [Probe] | 500 rxns |
RealHelix™ qRT-PCR Kit [Probe] is a convenient and reliable one-step quantitative RT-PCR kit. Both cDNA synthesis and PCR reactions are performed in a single tube using sequence-specific primers corresponding to the target RNAs from total RNA or mRNA. The Enzyme Mix in this kit is an optimized blend of HelixCript™ Thermo Reverse Transcriptase, RNase inhibitor and a specially designed automatic hot-start version of PCR enzyme, and ensures reliable results in terms of sensitivity, accuracy and specificity. The 2x Buffer Mix contains all of the required components including optimized buffer components, Mg2+, dNTPs. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal.
![Figure 1. Multiplex (four targets) qRT-PCR with RealHelix™ qRT-PCR Kit [Probe].](/upload_files/editor/20190221141558219.jpg "QRT2-P_Figure 1")
![Figure 2. Comparison of RealHelix™ qRT-PCR Kit [Probe] and qRT-PCR Kit [Probe type] (the previous version).](/upload_files/editor/2019022114205217.jpg "QRT2-P_Figure 2")