HelixAmp™ Hot-Start Fidelity Polymerase is a hot-start formulation of a modified Pfu DNA polymerase, meticulously optimized for robust, high-fidelity, and specific amplification of DNA fragments up to 5kb in length. This high-fidelity enzyme demonstrates a 2.5-fold increase in fidelity compared to conventional Pfu DNA polymerase and 100-fold higher than Taq DNA polymerase in accuracy. The provided 5x HSF buffer (a reaction buffer) includes all essential PCR components, such as buffering reagents, magnesium, salts, and dNTPs. Additionally, the supplied TuneUp™ Solution is for the challenging amplifications of templates with high G+C content or structural complexities, such as repeat sequences.

Concentration

• Hot-Start Fidelity Polymerase : 2.5 units/μl

Storage

• Store below -20 ℃

Shelf life

• 12 months

Storage buffer

• 50 mM Tris-HCl (pH 8.0)
• 100 mM KCl
• 0.1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol