HelixCript™ One-Step RT-PCR Kit [Speed-Pfu]는 total transcripts로부터 한 튜브 반응으로 타겟 유전자를 높은 sensitivity로 증폭하도록 디자인 된 제품입니다. 본 제품에 포함된 Enzyme Mix에는 HelixCript™ Thermo Reverse Transcriptase, HelixAmp™ Speed-Pfu Polymerase, 및 RNase inhibitor가 혼합되어 있습니다. 2x Reaction Mix에는 buffer, MgCl₂ 및 dNTP를 혼합/최적화하였습니다. 

Figure 1. A fast RT-PCR using HelixCript™ One-Step RT-PCR Kit [Speed-Pfu]. One-Step RT-PCR reactions were performed under the modified protocol as follows : The total time required for a One-Step RT-PCR reaction is 1 hour. One-step RT-PCRs were perfomed with 1 to 100 ng of human blood total RNA using HelixCript™ One-Step RT-PCR Kit [Speed-Pfu] and the primer set for human β-globin. The PCR reaction was carried out at 95℃(10 sec), 55℃(10 sec), and 72℃(20 sec) for 35 cycles.

Storage

• -20 ± 5 ℃

Shelf life

• 12 months