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RealHelix™ qRT-PCR Kit [v6a] [UDG System]은 최대 5개의 서로 다른 target RNA를 한 번의 반응으로 신속하게 검출하도록 최적화된 설계된 probe 기반 multiplex qRT-PCR 제품입니다(고속 증폭: 45 분/40 cycles 또는 초고속 증폭: 25 분/40 cycles). Enzyme mix는 역전사효소(reverse transcriptase), antibody-mediated hot-start PCR 중합효소, RNase inhibitor 및 heat-labile uracil-DNA glycosylase(HL-UDG)가 최적화된 조성으로 혼합되어 있으며, 5x reaction buffer에는 Mg²⁺, dNTP/dUTP를 포함한 필수 요소들이 포함되어 있습니다. 본 제품은 빠른 PCR 속도와 더불어 높은 sensitivity, specificity 및 정확도(accuracy)를 보장합니다. UDG system을 적용하여, carryover contamination을 효과적으로 방지할 수 있습니다.

 

Figure 1. Multiplex qRT-PCR using the RealHelix™ qRT-PCR Kit [v6a] [UDG System].

10-fold dilutions of purified SARS-CoV-2 viral RNA (from 10^-4 to 10^-7) spiked into a swab sample were analyzed using three different sets of target-specific primers and probes (N gene, ORF8, and S gene). The amount of viral RNA used in the 10^-7 dilution fold corresponds to approximately 10² copies. The probes were labeled with Texas Red (N gene), JOE (ORF8), FAM (S gene). The multiplex reaction was tested using the RealHelix™ qRT-PCR Kit [v6a] [UDG System] on a real-time PCR instrument, Bio-Rad CFX96.

 

 

 

 

Figure 2. Multiplex qRT-PCR using the RealHelix™ qRT-PCR Kit [v6a] [UDG System].

Plasmid clone mixtures harboring the partial region for each SARS-CoV-2 were used as templates in this reaction. SARS-CoV-2 clone mixture (10⁶, 10⁵, 10⁴, 10³, 10² copies) were analyzed using three different sets of target-specific primers and probes (N gene, ORF8 and S gene). The probes were labeled with Texas Red (N gene), JOE (ORF8), and FAM (S gene). The multiplex reaction was tested on a real-time PCR instrument, Bio-Rad CFX96.

Storage

• Store below -20 ℃

 

Shelf life

• 12 months