본 제품은 HelixAmp™ Premium-Taq Polymerase의 bulk 공급 품목입니다.
대량구매를 원하시는 경우 sale@nanohelix.net으로 문의주시기 바랍니다.
HelixAmp™ Premium-Taq Polymerase는 Ab+Taq polymerase에 ㈜나노헬릭스의 PMT(Polymerase Modulator on Temperature) 기술이 적용된 효소로, specificity가 매우 높은 PCR 증폭이 가능합니다. 본 효소에 결합된 anti-Taq antibody는 초기 변성 단계(pre-denaturation step)에 도달할 때까지 효소 활성을 억제시키며, PMT 기술이 적용된 buffer는 PCR 과정에서 primer dimer 형성을 억제하고 비특이적 증폭을 감소시킵니다.
Bulk 공급 품목은 enzyme만 제공되며, 5x Reaction Buffer 및 기타 구성품은 요청 시 별도 제공됩니다.
Figure 1. Comparison of HelixAmp™ Premium-Taq Polymerase with other company’s hot start versions of DNA polymerases.
12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under various annealing temperatures. A : Premium-Taq [NanoHelix], B : Company I, C : Company I, D : Company T.
Figure 2. Superior performance of HelixAmp™ Premium-Taq Polymerase on difficult targets.
Different primer sets (ApoE, MD, Prion, COMT) designed from the indicated region of the human genome were used in this PCR. PCRs were performed using 10 ng of human genomic DNA. All of the DNA polymerases are hot-start versions of each brand. A: Company T, B: Company I, C: Company B, D: Company I, E: Company S, F: Premium-Taq [NanoHelix].
Enzyme activities: Highly processive 5'-3' DNA polymerase; double-strand specific 5'-3' exonuclease; no 3'-5' exonuclease activity
Enzyme activation: 2 min at +95 ℃
Storage buffer: 20 mM Tris-HCl (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers and 50 % (v/v) glycerol
DNase contamination test: Not detectable (Incubation with 40 U enzyme and pUC19 plasmid at 37 ℃, 1 hr)
RNase contamination test: Not detectable (Incubation with 40 U enzyme and Human total RNA at 37 ℃, 1 hr)
Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)
Stability: 12 months at -20 ℃
