DNA-RNA Amplification
PCR Enzymes
Routine PCR
제품문의
Taq Polymerase
Strong heat-resistant enzyme with robust and efficient amplification.
- 일반적인 PCR
- 높은 증폭률(productivity)와 민감도(sensitivity)
- TA cloning
Products
| Cat.No. |
Product |
Size |
Price(KRW) |
| T500 |
HelixAmp™ Taq Polymerase |
500 units |
85,000 |
| T2500 |
HelixAmp™ Taq Polymerase |
2,500 units |
340,000 |
| T500N |
HelixAmp™ Taq Polymerase (with dNTP) |
500 units |
95,000 |
| T2500N |
HelixAmp™ Taq Polymerase (with dNTP) |
2,500 units |
380,000 |
| TBF500 |
HelixAmp™ Taq Polymerase [MgCl₂ free] |
500 units |
85,000 |
| TBF2500 |
HelixAmp™ Taq Polymerase [MgCl₂ free] |
2,500 units |
340,000 |
| TBF500N |
HelixAmp™ Taq Polymerase [MgCl₂ free] (with dNTP) |
500 units |
95,000 |
| TBF2500N |
HelixAmp™ Taq Polymerase [MgCl₂ free] (with dNTP) |
2,500 units |
380,000 |
HelixAmp™ Taq Polymerase는 Thermus aquaticus DNA polymerase 유전자를 박테리아 숙주 세포에서 발현/정제한 재조합 효소입니다. 본 제품은 thermostability와 sensitivity가 향상되도록 설계된 Taq DNA 중합효소로, 나노헬릭스의 고유한 공정 과정을 통해 높은 순도로 정제되었으며, 일반적인 PCR에 적합합니다. 본 제품에는 최고의 결과를 도출하기 위해 고순도 dNTP와 TuneUp™ Solution이 포함되어 있습니다. TuneUp™ Solution은 G+C 비율이 높거나 복잡한 구조의 target을 효율적으로 증폭할 수 있도록 도와줍니다.
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Figure 1. The purity of HelixAmp™ Taq polymerase. The purity and concentration of the prepared enzyme were analyzed by SDS-PAGE with each unit represented. St: Standard Protein marker. |
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Figure 2. The sensitivity of HelixAmp™ Taq polymerase. HelixAmp™ Taq polymerase amplified targets from the genomic region of human PKD (polycystic kidney disease) gene at various concentrations of human genomic DNA. 1.25U of Taq DNA polymerases manufactured by NanoHelix and other companies were used. |
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Figure 3. Comparison of the PCR performances of commercial Taq polymerases on various sizes of targets. The PCRs were performed with 1.25 units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions. |
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| Figure 4. Superior activity of HelixAmp™ Taq polymerase. The PCRs were performed with indicated units of Taq polymerases and supplied buffers from each manufacturer. All other components and conditions applied are identical throughout the reactions. NC, negative control reactions were performed with 2.5 units of enzymes without template DNA. |
Concentration
- Taq Polymerase : 5 units/μl
Storage
Shelf life
Storage buffer
- 20 mM Tris-HCl (pH 9.0)
- 100 mM KCl
- 0.1 mM EDTA
- 1 mM DTT
- Stabilizers
- 50 % Glycerol
