DNA/RNA Amplification Isothermal Amplification
Genome Amplifier (WGA)
- Fast and uniform amplification across entire genome within 90 min.
- Multiple Displacement Amplification by Phi29 DNA polymerase
- Whole genome amplification from as little as 10 ng of template DNA.
- More than 10 ㎍ of DNA products in a reaction
|HGA001-50||HelixAmp™ Genome Amplifier (WGA)||50 rxns|
|HGA001-100||HelixAmp™ Genome Amplifier (WGA)||100 rxns|
HelixAmp™ Genome Amplifier is a whole genome amplification kit suitable to amplify the whole genome from very little amount of genomic DNA sample. The whole genome amplification using HelixAmp™ Genome Amplifier is performed by Phi29 DNA polymerase with an isothermal MDA (multiple displacement amplification) based mode. Because of the strand displacement activity, strong 3’→5’ exonuclease activity and extreme processivity, Phi29 DNA polymerase could produce DNA up to 100 kb long with high fidelity. About 1,000 fold amplified DNA can be obtained from 10 ng genomic DNA within 90 min using this kit. The amplified genome can be directly applied, without further purification, to the downstream genetic analysis works including PCR, genotyping, and library constrictions.