Raw Materials for MDx Conventional PCR/RT-PCR premixes

One-Step RT-PCR Kit [Hot-Taq]

  • Fast and easy one-tube setup 
  • Convenient enzyme mix and 2x reaction mix
  • Heat-labile UDG system: prevention of carryover contamination
  • Convenience and reduced contamination risk
  • The highest specificity of PCR amplification 


Cat.No. Product Feature Size
BORTH One-Step RT-PCR Kit [Hot-Taq] - Custom
BORTHU One-Step RT-PCR Kit [Hot-Taq], w/UDG + UDG SYSTEM* Custom

One-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reactions from RNA templates. Thermo Reverse Transcriptase, Hot-Taq polymerase, and RNase Inhibitor comprise the enzyme mixture.

An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs. Optional UDG system includes the Heat-labile UDG and dUTP.




  • Viral RNA detection
  • Gene-expression analysis
  • Molecular Diagnosis
  • Endpoint one-step RT-PCR from any RNA transcript


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