Thermo Reverse Transcriptase, a thermostable and RNase H-negative variant of M-MLV Reverse Transcriptase (RTase), is an enzyme to synthesize cDNAs from RNA templates at a temperature range of 42℃ ~ 55℃ and shows the highest activity at 50℃. The high processivity and productivity of Thermo Reverse Transcriptase can synthesize cDNA up to 12 kb from an RNA template.

Application 

• Generation of the first-strand cDNA up to 12 kb for libraries or cloning
• Two-step or One-step RT-PCR applications 
• Conventional or real-time RT-PCR 
• RT-PCR for detection of viral RNA

Enzyme activities: RNA-dependent DNA polymerase. No RNase H activity 

Recommended reaction temperature: 42 to 65°C 

Storage buffer: 20mM Tris-HCl (pH7.5), 0.1mM EDTA-NaOH (pH 8.0), 100mM KCl, 0.01% Nonidet P-40, 1mM DTT, 50% Glycerol

Purity: ≥90% (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 200U enzyme and pUC19 plasmid at 37°C, 1hr) 

RNase contamination test: Not detectable (Incubation with 200U enzyme and Human total RNA at 37°C, 1hr)

Activity test in cDNA synthesis, RT-PCR and qRT-PCR: Correspond to reference (Human RNA target)

Stability: 24 months at -20°C.