DNA-RNA Amplification PCR Enzymes Fidelity PCR
Hot-Start Fidelity Polymerase
Upgraded proof-reading activity, maximized productivity, even with difficult targets.
- Chemically-modified hot-start Pfu polymerase
: Suppresses 3'-5' exonuclease activity at room temperature. Convenient PCR mixture setup. - High fidelity: 2.5 times higher than Pfu polymerase, 100 times higher than Taq polymerase
- High specificity: Minimizes non-specific amplification and primer-dimer formation
- High-yield amplification of challenging targets (e.g., GC-rich)
Products
| Cat.No. | Product | Size |
|---|---|---|
| HSF250 | HelixAmp™ Hot-Start Fidelity Polymerase | 250 units |
HelixAmp™ Hot-Start Fidelity Polymerase is a hot-start formulation of a modified Pfu DNA polymerase, meticulously optimized for robust, high-fidelity, and specific amplification of DNA fragments up to 5kb in length. This high-fidelity enzyme demonstrates a 2.5-fold increase in fidelity compared to conventional Pfu DNA polymerase and 100-fold higher than Taq DNA polymerase in accuracy. The provided 5x HSF buffer (a reaction buffer) includes all essential PCR components, such as buffering reagents, magnesium, salts, and dNTPs. Additionally, the supplied TuneUp™ Solution is for the challenging amplifications of templates with high G+C content or structural complexities, such as repeat sequences.
Manual