HelixAmp™ HyperSense-Taq Polymerase is the most suitable enzyme for the detection of extremely low copy target DNA. In a PCR reaction with HelixAmp™ HyperSense-Taq Polymerase 1 ~ 5 copies of target could be detected. This robust polymerase is made of sophisticated blending of Taq DNA polymerase, a proof-reading DNA polymerase, anti-Taq antibody and noble additional factors to enhance the specificity and productivity of PCR amplification. HelixAmp™ HyperSense-Taq Polymerase is also applicable to long-range PCR up to 20 kb from genomic DNA. HelixAmp™ HyperSense-Taq Polymerase shows 4 to 5 times of higher fidelities than normal Taq DNA polymerases. The addition of TuneUp™ Solution in the reaction mixture is a simple way to optimize the long-range PCR or difficult targets of PCR.

 

 

Figure 1. HelixAmp™ HyperSense-Taq Polymerase possesses high sensitivity and productivity. In a PCR reaction with limited amount of templates (1 ng and 0.2 ng of human genomic DNA) and limited extension time (100 sec at 60 ℃), HelixAmp™ HyperSense-Taq Polymerase reveals superior performance compared to HelixAmp™ Xtender-Taq Polymerase. 

 

 

 

 

 

Figure 2. The extreme sensitivity of HelixAmp™ HyperSense-Taq Polymerase. The sensitivity of HelixAmp™ HyperSense-Taq Polymerase was evaluated by the amplification of beta-globin gene with 1.25 units of Xtender-Taq Polymerase or HyperSense-Taq Polymerase with various concentrations of template. Even from less than 5 copies (0.016 ng) of genomic DNA template, HelixAmp™ HyperSense-Taq Polymerase amplifies the target successfully. NC : Negative control without template.

Concentration

• HyperSense-Taq Polymerase : 2.5 units/μl

 

Storage

• -20 ± 5 ℃

 

Shelf life

• 24 months

 

Storage buffer

• 20 mM Tris-HCl (pH 9.0)
• 100 mM KCl
• 0.1 mM EDTA
• 1 mM DTT
• Stabilizers
• 50 % Glycerol