2012 Launching a new product #5
NanoHelix's Direct WGA Kit is a complete system for whole genome amplification from various tissues or samples directly without DNA purification processes. Very little amount of samples, several miligram or microliter volume, are required for the directly WGA. About 10 ug DNA products could be obtained in a standard reaction. The enzyme mix and buffer system are designed to tolerate against most amplification inhibitors found in crude samples. Phi29 DNA polymerase, the major polymerization enzyme of this kit, isothermally amplifies the genomic DNAs included in the samples with multiple displacement mechanism. Phi29 DNA polymerase could produce DNA strand up to 70 kb long with high fidelity. All required components including enzymes, buffers, dNTPs, random primers, and sample pretreatment reagents are supplied in this kit. The amplified DNA products could be applied for successive PCR, genotyping, and library construction.