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PMT technology
Hit 3111
Date 2012.07.16

 

NanoHelix's New Technology

 

 



Polymerase Modulator on Temperature(PMT) technology, a noble PCR technolgy for high specific amplification and minimize the primer dimer formation.

PMT, a chemical, is regarded to interact with the amino groups of Taq DNA polymerase and reducing its affinity to DNA and polymerization activity.

The ionic status of PMT is altered by temperature and accordingly it is released from Taq DNA polymerase at higher temperature, above 50 . This temperature dependent inhibition of PCR enzyme by PMT results high specific target amplification and minimizes the primer dimer production.

In conjunction with classical antibody-mediated hot start methods, PMT maximizes the hot start PCR effects.

This noble method, developed by NanoHelix Co. Ltd. is applied to NanoHelix's special enzymes and kits.

The PMT applied PCR enzymes amplify target DNAs at broad-range of annealing temperature and exert higher sensitivity and specificity than other conventional PCR enzymes.