● Fast and reliable quantification of target DNA
● High specific amplification using hot-start PCR enzyme.
● Contains a strong fluorescent dye.
● ROX passive reference dye provided.?
The RealHelix™ qPCR Kit, 2X Pre-Mix, [Probe Type]?(Ver 2.0) is designed to perform a rapid real-time quantification of target DNA or 1st-strand cDNA using dual-labeled probe. The kit consists of convenient 2x concentrated premix solution and ROX passive dye. The 2x premix contains hot-start PCR enzyme, dNTPs, buffers, Mg2+ and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the side products such as primer dimers. Included ROX dye could be used as a passive reference dye.
|◎ Quantification of target DNA sample by real-time PCR|
◎ 2x qPCR Premix
◎ ROX Passive Reference Dye
The RealHelix™ qPCR Kit, 2X Pre-Mix, [Probe Type]?(Ver 2.0) was evaluated by real-time PCR using the 10-fold serial-diluted human genomic DNA and a set of human gene-specific primer and dual-labeled probe with ABI 7900HT Fast-real-time PCR system (Applied Biosystems, USA) and Rotor-Gene Q instrument (QIAGEN, Germany).
Figure 1. Comparison of real-time PCR using RealHelix™ qPCR Kit [Intercalator type] and Maxima SYBR qPCR Master Mix (Company F). The reactions were anayzed using human genomic DNAs (100, 10, 1 ng) isolated from whole blood. The accuracy of real-time PCR is demonstrated by R² value = 0.9973 (RealHelix™ qPCR Kit) and R² value = 0.9964 (Maxima SYBR qPCR Master Mix).
Figure 2. Comparison of real-time PCR using ReaHelix™ qPCR Kit [Probe type] and TaqMan Fast Universal PCR Master Mix (Company I).Human genomic DNAs (100, 10, 1 ng) isolated from whole blood were analyzed using beta-globin DNA-specific primer set and 5’-FAM-labelled probe. The accuracy of real-time PCR is denomstrated by R² value = 0.99634 (RealHelix™ qPCR Kit) and R² value = 0.99917 (TaqMan Fast Universal PCR Master Mix).
Figure 3. Absolute quantification by RealHelix™ qPCR Kit. 10-fold serial-diluted human genomic DNA as the standard sample and 25 ng human genomic DNA as test sample were analyzed using the RealHelix™ qPCR Kit for estrogen receptor gene-specific primer set. The accuracy of real-time PCR using RealHelix™ qPCR Kit is demonstrated by R² value = 0.99649.
Figure 4. Molecular diagnostics using RealHelix™ qPCR Kit [Probe type]. Real-time PCR detection of Mycobacterium tuberculosis or Mycobacteria using qPCR Kit [Probe type].