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HelixAmp™ DNA-free Taq polymerase PCR enzymes Selection Guide    

● Host DNA-free
● Prevent false positive PCR from contaminating bacterial DNA
● Application in molecular diagnostics for bacterial pathogen
● Detection of bacteria by amplification of 16s rRNA region


Description

HelixAmp™ DNA-free Taq polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene and purified using a process that minimizes level of contaminating host DNA. HelixAmp™ DNA-free Taq polymerase is high quality DNA polymerase and suitable for molecular diagnostics. Because the contaminating host genomic DNA can cause the false positive results, use of DNA-free Taq polymerase is very important in PCR detection of bacteria with universal primers(e.g. 16s rRNA specific primers). A chemically modified hot-start version of this enzyme, HelixAmp™ DNA-free Hot-Taq polymerase, is also available. (see the product list)

Contents
◎ DNA-free Taq polymerase (5 units/㎕)
◎ 10X Reaction Buffer
◎ dNTP Mix (each 10 mM)
◎ 5X TuneUp™ solution

Quality Control
◎ Contamination assay for nucleases
◎ Contamination assay for host genomic DNA
◎ Activity and sensitivity assay

Storage buffer

50% Glycerol,20 mM Tris-HCl (pH9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1 mM PMSF


Data
 

 

Figure 1. HelixAmp™ DNA-free Taq polymerase was compared to Taq polymerse from competitors for performance and gDNA contamination by qPCR. HelixAmp™ DNA-free Taq (1.25U) was tested for presence of E. coli gDNA contamination in qPCR using 16s rRNA primers.

 


Figure 2. Comparison of conventional Taq DNA polymerase and HelixAmp™ DNA-free Taq polymerase for the contamination of host DNA. PCRs were performed with or without template. Primers are specific for the E. coli 16s rRNA-coding gene.

 


Figure 3. Comtamination test of host DNA in conventional Taq DNA polymerase(hot-start version) and HelixAmp™ DNA-free Taq polymerase(hot-start version) using a real-time PCR assay.

 

Cat.No. Product Size
 DFT250 DNA-free Taq polymerase 250 units 
 DFT250N DNA-free Taq polymerase (including dNTP Mix, 5x TuneUp™ solution) 250 units 
 DFT500 DNA-free Taq polymerase 500 units 
 DFT500N DNA-free Taq polymerase (including dNTP Mix, 5x TuneUp™ solution) 500 units 
 DFHT125 DNA-free Hot-Taq polymerase 125 units 
 DFHT125N DNA-free Hot-Taq polymerase (including dNTP Mix, 5x TuneUp™ solution) 125 units 
 DFHT250 DNA-free Hot-Taq polymerase 250 units 
 DFHT250N DNA-free Hot-Taq polymerase (including dNTP Mix, 5x TuneUp™ solution) 250 units