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HelixZyme™ RNase Inhibitor

● Inhibits common eukaryotic RNases
● Active over a broad pH range (pH 5~8)
● Direct use of RT-PCR and real-time RT-PCR


Description

HelixZyme™ RNase Inhibitor is recombinant protein of human placental RNase inhibitor expressed in E. coli and specifically inhibits major nonspecific ribonuclease including RNase A, B and C, This enzyme is in effective against RNase I, RNase T1, S1 nuclease, RNase H or RNase from Aspergilus. It is not observed inhibition of polymerase activity when RNase inhibitor is used with DNA polymerase, AMV or M-MuLV reverse transcriptase, or phage RNA polymerase(SP6, T7, or T3). This nature of RNase inhibitor is useful to protect RNA degradation during cDNA synthesis, RNA synthesis, RNA preparation, and so on.


Contents

RNase inhibitor (40 units/μl)


Application

RT-PCR
cDNA synthesis
In vitro transcription / translation
Enzymatic RNA labeling reaction

Cat.No. Product Size
 RNI2000 HelixZyme™ RNase Inhibitor (Recombinant) 2,000 units 
 RNI10K HelixZyme™ RNase Inhibitor (Recombinant) 10,000 units