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HelixZyme™ Uracil-DNA Glycosylase

- Thermo-labile version-
● Removing uracil-containing DNA
● Elimination of carryover contamination
● Engineered for heat susceptible
● Best for real-time PCR with dUTP


Description

NanoHelix Uracil-DNA-glycosylase(UDG), also known as UNG, is an engineered E. coli enzyme catalyzing the hydrolysis of N-glcosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. This enzyme has been engineered to be more susceptible to high-temperature and inactivated easily during the dUTP containing PCR or real-time PCR. The wild-type of E. coli UDG is resistant to thermal inactivation. For PCR diagnosis, UDG can be used for efficient removal of carryover contaminated uracil-containing DNA.


Application

◎ Elimination carryover contamination

 ◎ Genotyping and Molecular Diagnosis

 

Contents
Uracil-DNA glycosylase (1 unit/ul)

Quality control
Uracil-DNA glycosylase is evaluated by the removal of PCR product using dUTP with 1 unit of this enzyme.
Cat.No. Product Size
 UDG250 HelixZyme™ Uracil-DNA Glycosylase (UDG), thermolabile 250 units 
 UDG1000 HelixZyme™ Uracil-DNA Glycosylase (UDG), thermolabile 1,000 units 
 UDG50000 HelixZyme™ Uracil-DNA Glycosylase (UDG), thermolabile 5,000 units