● High fidelity DNA polymerase ● Strand-displacement synthesis ● High processivity up to 70 kb ● Best enzyme for RCA of circular DNA ● Used for WGA (whole genome amplification) and MDA (multiple displacement amplification) |
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Description |
NanoHelix Phi29 DNA polymerase is a recombinant protein purified from E. coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses the highest processivity and strand-displacement activity among the known DNA polymerase. |
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Application |
◎ Rolling-circle amplification (RCA) ◎ Multiple displacement amplification (MDA) ◎ Whole genome amplification (WGA) ◎ Preparation of DNA template for sequencing. |
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Contents |
Phi29 DNA polymerase (10 units/㎕) |
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