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PureHelix™ Total RNA Purification Kit

 

● High-yield and high-quality total RNA isolation
● Silica-membrane based
● Wide-range of applications


Description

PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] is designed for rapid, pure, and high yield isolation of total RNA from small amount of various samples, such as blood, animals, plants, bacteria, and viruses. This kit is suitable to the rapid preparation of nucleic acids for molecular diagnostics using conventional and real-time RT-PCR technologies. Due to no use of phenol, this kit is safe to handle and does not produce any harmful waste. Total RNA purified with this kit can be used in a number of downstream applications.


Application
◎ Preparation of total RNA for RT-PCR or quantitative RT-PCR
◎ Preparation of total RNA sample for molecular diagnostics

Quality Control

PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria] was tested for the isolation of total RNA from blood, animal tissue, plant leaf tissue and bacterial cell.


Data


Figure 1. Isolation of total RNA from animal or plant tissue samples using PureHelix™ Total RNA Purification Kit.Total RNAs were isolated from 30 mg of rat liver tissue, or 20 mg of tobacco leaf according to the manual. The extractions were duplicated and 1 μl from each 50 μl eluted solutions were analyzed on 1% agarose gel electrophoresis.

 



Figure 2. Real-time PCR analysis of Hepatitis A virus(HAV) for total RNAs purified from HAV-positive serum using PureHelix™ Total RNA Purification Kit. 10-fold serial-diluted total RNA purified from HAV-positive serum were analyzed using the RealHelix™ qRT-PCR Kit.


Figure 3. Viral RNA detection from total RNA isolated by PureHelix™ Total RNA Purification Kit. Total RNA was isolated from a nasopharyngeal specimen of new influenza virus-infected patient and one-step RT-PCR was performed for the target corresponding to each influenza A (InfA, 106 bp), Swine Influenza A(SW InfA, 195 bp), Swine Influenza H1(SW H1, 116 bp), or RNase P (internal control, 65 bp).
Cat.No. Product Size
 RPT50 PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria]
(Column Type)
50 preps 
 RPT100 PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria]
(Column Type)
100 preps 
 RPT200 PureHelix™ Total RNA Purification Kit [Virus, Animal, Plant, Bacteria]
(Column Type)
200 preps