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PureHelix™ PCR Purification Kit

● DNA clean-up
● Spin column based
● Easy removal of primer-dimers
● Recovery yield, >80%
● Sequencing grade


Description

PureHelix™ PCR Purification Kit is designed for rapid and high-yield clean-up DNA from PCR and other enzymatic reaction mixtures. PureHelix™ PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. The purification procedure removes primers, nucleotides, proteins, salts and other impurities from DNA samples. The high purity DNA isolated using this kit is adequate for any molecular biology applications. Especially, the purification of PCR products using the Dimer Removal condition for an efficient removal of primer dimers, by-products of PCR, ensures excellent results of the automating sequence analysis.


Application

DNA fragments purified with PureHelix™ PCR purification kit are ready for direct use in all application.
◎ Automatic fluorescent sequencing
◎ Restriction digestion
◎ Preparatation of radioactive labeled probe
◎ Ligation and transformation


Data
 

Figure 1. PCR purification of various sizes of PCR products using PureHelix™ PCR Purification Kit. C : PCR products before purification, Q : PCR product purified by QIAquick PCR Purification Kit. After 50 ul PCR product is finally eluted with 50 ul elution buffer, 10 % volume of each sample is electrophoresed in 1 % agarose gel.

 

 

Figure 2. PCR purification under High yield condition using PCR Purification Kit. C: PCR products (100 bp) before purification; Q: PCR product purified by QIAquick PCR Purification kit. PCR product was finally eluted with 50 μl elution buffer.

 

 

Figure 3. PCR purification under Dimer removal condition using PCR Purification Kit. B: PCR product mixture (100, 300, 600 bp) before purification; D: PCR product mixture purified under dimer removal condition; H: PCR product mixture purified under high yield condition.
Figure 4. Accurate Sequencing after purification. Sequence of a 1 kb PCR product purified by PCR purification kit. (A), O: PCR product derived from lamda DNA before purification, P: PCR product purified by PCR purification kit under dimer removal condition. (B) Sequencing analysis for PCR product purified by PCR purification kit.
Cat.No. Product Size
 PCR50 PureHelix™ PCR Purification Kit 50 preps 
 PCR200 PureHelix™ PCR Purification Kit 200 preps