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PureHelix™ Genomic DNA Prep Kit (Column Type)

● No phenol and chloroform
● Silica-membrane based
● High-purity isolation of genomic DNA
● High-throughput application


Description

PureHelix™ Genomic DNA Prep Kit (Column type) is designed for rapid and high purity isolation of genomic DNA from whole bloods, tissue culture cells, animal tissue, plant tissue, yeast, Gram-positive or Gram-negative bacteria. This kit is a spin column-based method and then can completely remove PCR inhibitors such as divalent cations and proteins and in results, obtain the high purity genomic DNA by the elution with low-salt solution or water. Due to no use of phenol, this kit is safe and does not produce any harmful waste. DNA purified with this kit is suitable for a variety of applications, including PCR amplification, digestion with restriction endonucleases and membrane hybridizations.


Application

◎ PCR, quantitative, real-time PCR
◎ Southern blotting analysis.
◎ Genotyping
◎ Discovery and validation of biological markers (SNP or SSR)


Data



Figure 1. Isolation of genomic DNA from various samples using Genomic DNA prep Kit (Column Type). Isolation of genomic DNA from 500 μl liquid-cultured E. coli (2 X 109 cell/ml) and Pepper leaf tissue, Cow muscle, Human Blood. Purification of genomic DNAs was performed following manufacturer’s manual.

 
Figure 2.Typical yields of pure genomic DNA obtained from various animal or plant samples. N: Genomic DNA purified by PureHelix™; Q: by DNeasy®. Isolation of genomic DNA from 80 mg of various tissues. Purification of genomic DNAs was performed following manufacturer’s manual. (A), Animal tissues. (B), Plant tissues, Gm: Glycine max; Os:, Orysa sativa; Nt: Nicotiana tabacum; Oc: Oriental cherry; Ca: Capsicum annuum; Sl: Solanum lycopersicum.
Cat.No. Product Size
 GCT50 PureHelix™ Genomic DNA Prep Kit [Blood, Animals, Plants] (Column Type) 50 preps 
 GCT100 PureHelix™ Genomic DNA Prep Kit [Blood, Animals, Plants] (Column Type) 100 preps 
 GCT200 PureHelix™ Genomic DNA Prep Kit [Blood, Animals, Plants] (Column Type) 200 preps 
 GCB50 PureHelix™ Genomic DNA Prep Kit [Bacteria] (Column Type) 50 preps 
 GCB100 PureHelix™ Genomic DNA Prep Kit [Bacteria] (Column Type) 100 preps 
 GCB200 PureHelix™ Genomic DNA Prep Kit [Bacteria] (Column Type) 200 preps 
 GCY50 PureHelix™ Genomic DNA Prep Kit[Yeast] (Column Type) 50 preps 
 GCY100 PureHelix™ Genomic DNA Prep Kit[Yeast] (Column Type) 100 preps 
 GCY200 PureHelix™ Genomic DNA Prep Kit[Yeast] (Column Type) 200 preps 
 GCTN50 PureHelix™ Genomic DNA Prep Kit [Bacteria, Animals, Plants] (Column Type) 50 preps 
 GCTN100 PureHelix™ Genomic DNA Prep Kit [Bacteria, Animals, Plants] (Column Type) 100 preps 
 GCTN200 PureHelix™ Genomic DNA Prep Kit [Bacteria, Animals, Plants] (Column Type) 200 preps 
 GCBL50 PureHelix™ Genomic DNA Prep Kit [Blood or Animal cells] (Column Type) 50 preps 
 GCBL100 PureHelix™ Genomic DNA Prep Kit [Blood or Animal cells] (Column Type) 100 preps 
 GCBL200 PureHelix™ Genomic DNA Prep Kit [Blood or Animal cells] (Column Type) 200 preps