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HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)

● Multiplex PCR up to 13-plex
● Allele specific multiplex PCR
● Automatic hot start PCR
● Extreme Specificity


Description

HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0), the best choice to setup complex multiplexing PCR, is a pre-mixed solution containing HelixAmp™ Hot Taq DNA polymerase, dNTPs and optimized buffer as 2x concentration. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)is designed for hot-start PCR to allow the highest specificity and minimize the interruptions among the primers in a reaction mixture. This pre-mixed enzyme is highly suitable for up to 13-plex PCR, allele specific PCR (discriminating SNP), and applications for genotyping. The provided TuneUp™ solution helps to improve the yield and specificity in problematic PCRs. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)provides the most suitable condition for efficient and reproducible multiplex or specific PCR.


Application
◎ Multiplex PCR
◎ Allele specific PCR

Contents

HelixAmp™ Ready-2x-MultiPlex PCR Master Mix (Ver. 2.0)containing HelixAmp™ Hot Taq DNA polymerase (Ver. 2.0), Buffer, dNTPs and stabilizing agents.


Quality Control

HelixAmp™ Ready-2x-Go series is evaluated by amplification compare with mixture of each component required in PCR for various size of target without further optimization at a different concentration of template DNA.


Data
Figure 1. Multiplex PCR of up to 8 targets using HelixAmp™ Ready-2x-MultiPlex. Multiplex PCR in single-tube reaction is performed with rice genomic DNA and mixture of allele-specific primer set corresponding to SNP. Primer set corresponding to each target was added into one-tube multiplex PCR one by one. M: Standard size marker

Figure 2. Multiplex PCRs for various multiplex primer sets using HelixAmp™ Ready-2x-MultiPlex. Q : QIAGEN multiplex PCR premix, N : NanoHelix’s HelixAmp™ Ready-2x-MultiPlex.


Cat.No. Product Size
 MU001S HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (withuot dye) 1.25 ml/tube x 2ea 2.5 ml 
 MU001L HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (withuot dye) 1.25 ml/tube x 10ea 12.5 ml 
 MUD001S HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) 1.25 ml/tube x 2ea 2.5 ml 
 MUD001L HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) 1.25 ml/tube x 10ea 12.5 ml 
 MUT001-96 HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (without dye) 8-Strip x 12 / Plate 96 tests 
 MUDT001-96 HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) 8-Strip x 12 / Plate 96 tests 
 MUUD01-M1 HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) [UDG system], 1 ml/tube 1 ml 
 MUUD01-M5 HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) [UDG system], 1 ml/tube x 5 tubes 5 ml 
 MUUD01-M50 HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) [UDG system] 1 ml/tube x 50 tubes 50 ml 
 MUUD01-M50B HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0) (with dye) [UDG system] 50 ml / Bottle 50 ml