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HelixAmp™ Xtender-Taq Polymerase PCR enzymes Selection Guide    
● Long-range PCR up to 20 kb
● Higher fidelity than Taq DNA polymerase
● High productivity and sensitivity
● TA cloning

Description

HelixAmp™ Xtender-Taq polymerase is very suitable and ideal for long-ranged PCR up to ~40kb from isolated viral DNAs or up to ~20kb from genomic DNAs. This enzyme mixture is an optimized blend of HelixAmp™ Taq polymerase and HelixAmp™ Power-Pfu polymerase. Due to the proofreading activity of HelixAmp™ Power-Pfu polymerase, HelixAmp™ Xtender-Taq polymerase possesses the greater yield, processivity and fidelity than Taq DNA polymerase alone. The fidelity of HelixAmp™ Xtender-Taq polymerase is higher approximately 4 times than that of HelixAmp™ Taq polymerase. The high productivity of HelixAmp™ Xtender-Taq polymerase is suited to the amplification of a low copy target DNA. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the long-range PCR.


Application

◎ Routine PCR
◎ Long range PCR
◎ Generation of PCR products for TA cloning


Contents

◎ HelixAmp™ Xtender-Taq polymerase (2.5 units/)
◎ 10X reaction buffer
◎ dNTP mix (each 10 mM)
◎ 5X TuneUp™ solution


Quality Control

◎ Activity assay
◎ Sensitivity assay


Storage buffer
50% Glycerol, 20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1mM PMSF

Data
Figure 1. HelixAmp™ Xtender-Taq polymerase successfully amplifies up to 20 kb products from a genomic DNA. Each target of genomic DNA was amplified from 50 ng of human genomic DNAs. TuneUp™ solution was used to optimize the amplification of each target. lane 1 : 1 kb, lane 2 : 3.18 kb, lane 3 : 8 kb, lane 4 : 14 kb, lane 5 : 17 kb, lane 6 : 20 kb, lane 7 : 22 kb. M1 : HelixRuler™ 1kb(+) DNA ladder, M2 : λ DNA/HindIII.

Figure 2. HelixAmp™ Xtender-Taq polymerase is faster and more productive than Taq DNA polymerase. The higher speed and productivity of HelixAmp™ Taq polymerase represents more products in a reaction of limited extension time (30 sec/kb).
Cat.No. Product Size
 XT250 HelixAmp™ Xtender-Taq polymerase (including 10x Reaction Buffer) 250 units 
 XT250N HelixAmp™ Xtender-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
250 units 
 XT500 HelixAmp™ Xtender-Taq polymerase (including 10x Reaction Buffer) 500 units 
 XT500N HelixAmp™ Xtender-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 XT2500 HelixAmp™ Xtender-Taq polymerase (including 10x Reaction Buffer) 2,500 units 
 XT2500N HelixAmp™ Xtender-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
2,500 units