Home > Products > DNA / RNA Amplification > PCR enzymes
 

HelixAmp™ Taq Polymerase PCR enzymes Selection Guide    
● High productivity
● Routine PCR
● TA cloning

Description
HelixAmp™ Taq polymerase is a recombinant enzyme expressed and purified from a bacterial host cell harboring Thermus aquaticus DNA polymerase gene. HelixAmp™ Taq polymerase is an engineered Taq DNA polymerase enforced its thermostablity and template sensitivity. This highly purified thermostable DNA polymerase with unique NanoHelix’s purification process is quite suitable for routine PCR. For the maximum performance extremely pure dNTPs and TuneUp™ solutions are also included. TuneUp™ solution helps HelixAmp™ Taq polymerase to efficiently amplify the problematic target region of high G+C content or structural problem

Contents
◎ HelixAmp™ Taq polymerase (5 units/㎕)
◎ 10X Reaction Buffer
◎ dNTP Mix (each 10 mM)
◎ 5X TuneUp™ solution

Quality Control
◎ Contamination assay for nucleases (exo- and endo-)
◎ Contamination assay for bacterial host DNA
◎ Activity assay
◎ Sensitivity assay
◎ Protein purity analysis

Storage buffer
50% Glycerol,20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1mM PMSF

Data
Figure 1. The purity of HelixAmp™ Taq polymerase. The purity and concentration of the prepared enzyme were analyzed by SDS-PAGE with each units represented. St: Standard Protein marker.

Figure 2. Sensitivity of HelixAmp™ Taq polymerase. HelixAmp™ Taq polymerase amplified target from genomic region of human PKD (polycystic kidney disease) gene at various concentrations of human genomic DNA. 1.25U of Taq DNA polymerases manufactured by NanoHelix and other companies were used.

Figure 3. Comparison of the PCR performances of commercial Taq polymerases on various sizes of targets. The PCRs were performed with 1.25 units of Taq polymerases and supplied buffers from each manufactures. All other components and conditions applied are identical through the reactions.

Figure 4. Superior activity of HelixAmp™ Taq polymerase. The PCRs were performed with indicated units of Taq polymerases and supplied buffers from each manufactures. All other components and conditions applied are identical through the reactions. NC, negative control reactions were performed with each 2.5 units of enzymes without template DNA.
Cat.No. Product Size
 T500 HelixAmp™ Taq polymerase (including 10x Reaction Buffer) 500 units 
 T500N HelixAmp™ Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 T2500 HelixAmp™ Taq polymerase (including 10x Reaction Buffe, Blue Box) 2,500 units 
 T2500N HelixAmp™ Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units 
 TBF500 HelixAmp™ Taq polymerase (including 10x Mg-free buffer, 25mM MgCl₂) 500 units 
 TBF500N HelixAmp™ Taq polymerase
(including 10x Mg-free buffer, 25mM MgCl₂, TuneUp™ solution, dNTP Mix)
500 units 
 TBF2500 HelixAmp™ Taq polymerase (including 10x Mg-free buffer, 25mM MgCl₂, Blue Box) 2,500 units 
 TBF2500N HelixAmp™ Taq polymerase
(including 10x Mg-free buffer, 25mM MgCl₂, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units