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HelixAmp™ Speed-Pfu Polymerase PCR enzymes Selection Guide    
● Highest fidelity (15 ~ 20 times higher fidelity than Taq)
● Express speed. Fast PCR ( <30 sec/kb extension )
● Long-range PCR, up to 20kb from genomic DNA
● High productivity

Description

HelixAmp™ Speed-Pfu polymerase is an editorial enzyme improved from HelixAmp™ Power-Pfu polymerase. HelixAmp™ Speed-Pfu polymerase shows faster (4~6 times) polymerization reaction and higher fidelity (3~5 times) than any other high-fidelity enzymes. With this high speed and accuracy, HelixAmp™ Speed-Pfu polymerase is most suitable to faithful amplification of relatively long-ranged target for cloning etc. Due to its high speed, fast PCR with this enzyme could be completed in 30 min for the reliable amplification of less than 1 kb size target DNA. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.


Application

◎ High fidelity PCR
◎ Long range PCR
◎ Site-directed mutagenesis
◎ Blunt end PCR cloning


Contents

HelixAmp™ Speed-Pfu polymerase (2.5 units/)
10X Reaction buffer
dNTP mix (each 10 mM)
5X TuneUp™ solution


Quality Control

◎ Contamination assay for bacterial host DNA
◎ Activity assay
◎ Sensitivity assay


Storage buffer
50% Glycerol, 50mM Tris-HCl (pH8.0), 0.1mM EDTA, 1mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 1mM PMSF

Data
 

Figure 1. The express speed of HelixAmp™ Speed-Pfu polymerase. Four different sizes of human gene fragments were amplified with various extension times. PCR reactions were carried out at 95, 10 sec and 68for the represented extension time (30 cycles). This result indicates that only 20-23 sec is required for successful synthesis of 1kb size DNA. The total PCR time required for amplification of 1 kb fragment is less than 25 minutes.


Figure 2. A fast PCR with HelixAmp™ Speed-Pfu polymerase and other enzymes. 1 kb fragment of human DNA was amplified from various concentrations of template in a limited extension time, 20 sec. PCR reaction was carried out at 95, 10 sec and 68, 20 sec (35 cycles).


Figure 3. HelixAmp™ Speed-Pfu polymerase is the enzyme with the highest fidelity. Error Rate = mutation/bp/duplication

Cat.No. Product Size
 S250 HelixAmp™ Speed-Pfu polymerase (including 10x Reaction Buffer) 250 units 
 S250N HelixAmp™ Speed-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
250 units 
 S500 HelixAmp™ Speed-Pfu polymerase (including 10x Reaction Buffer) 500 units 
 S500N HelixAmp™ Speed-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 S2500 HelixAmp™ Speed-Pfu polymerase (including 10x Reaction Buffer, Blue Box) 2,500 units 
 S2500N HelixAmp™ Speed-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units