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HelixAmp™ Power-Pfu Polymerase (Ver. 2.0) PCR enzymes Selection Guide    
● High fidelity and productivity
● PMT (Polymerase Modulator on Temperature) technology
● Broad range of annealing temperature
● Minimize the non-specific amplification

Description

HelixAmp™ Power-Pfu polymerase (ver. 2.0), originated from an extreme thermophile, is ideal for high-fidelity PCR amplification. Due to its intrinsic 3’ to 5’ exonuclease (proof-reading) activity, this DNA polymerase shows high-fidelity and produces blunt ended PCR products. NanoHleix's PMT(Polymerase modulator on temperature) technology" is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR.HelixAmp™ Power-Pfu polymerase (ver. 2.0)is an engineered enzyme showing much higher productivity and processivity than other proof-reading thermo-stable enzymes (e.g. Pfu DNA polymerase, Vent DNA polymerase etc.) without compromise to the fidelity. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.


Application

◎ High fidelity PCR
◎ Site-directed mutagenesis
◎ Blunt end PCR cloning


Contents

◎ HelixAmp™ Power-Pfu polymerase (2.5 units/)
◎ 10X Reaction buffer
◎ dNTP mix (each 10 mM)
◎ 5X TuneUp™ solution


Quality Control

◎ Contamination assay for bacterial host DNA
◎ Activity assay
◎ Sensitivity assay
◎ Protein purity analysis


Storage buffer
50% Glycerol, 50mM Tris-HCl (pH8.0), 0.1mM EDTA, 1mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 1mM PMSF

Data

 


 

Figure 1. Various annealing temperature of HelixAmp™ Power-Pfu polymerase. 12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under the various annealing temperature. AT = annealing temperature.


Figure 2. The fidelities of PCR enzymes. The fidelity of HelixAmp™ Power-Pfu polymerase was compared with high fidelity enzymes from other companies and Taq polymerase. Error rate = mutation/bp/duplication.
Cat.No. Product Size
 P250 HelixAmp™ Power-Pfu polymerase (including 10x Reaction Buffer) 250 units 
 P250N HelixAmp™ Power-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
250 units 
 P500 HelixAmp™ Power-Pfu polymerase (including 10x Reaction Buffer) 500 units 
 P500N HelixAmp™ Power-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 P2500 HelixAmp™ Power-Pfu polymerase (including 10x Reaction Buffer, Blue Box) 2,500 units 
 P2500N HelixAmp™ Power-Pfu polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units