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HelixAmp™ HyperSense-Taq Polymerase PCR enzymes Selection Guide    
● Extreme sensitivity on low copy of templates
● Automatic hot-start PCR
● Long-range PCR
● Higher fidelity than Taq DNA polymerase
● High productivity

Description

HelixAmp™ HyperSense-Taq polymerase is the most suitable enzyme for the detection of extremely low copy target DNA. In a PCR reaction with HelixAmp™ HyperSense-Taq polymerase 1-5 copies of target could be detected. This robust polymerase is made of sophisticated blending of Taq DNA polymerase, a proof-reading DNA polymerase, anti-Taq antibody and noble additional factors to enhance the specificity and productivity of PCR amplification. HelixAmp™ HyperSense-Taq polymerase is also applicable to long-range PCR up to 20 kb from genomic DNA. HelixAmp™ HyperSense-Taq polymerase shows 4 to 5 times of higher fidelities than normal Taq DNA polymerases. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the long-range PCR or difficult targets of PCR.


Application

◎ Hot start PCR
◎ Routine PCR
◎ Long range PCR
◎ Generation of PCR products for TA cloning


Contents

◎ HelixAmp™ HyperSense-Taq polymerase (2.5 units/)
◎ 10X Reaction buffer
◎ dNTP mix (each 10 mM)
◎ 5X TuneUp™ solution


Quality Control

◎ Activity assay
◎ Sensitivity assay


Storage buffer
50% Glycerol, 20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1mM PMSF

Data
Figure 1. HelixAmp™ HyperSense-Taq polymerase possesses the high sensitivity and high productivity. In a PCR reaction with limited amount of templates (1 ng and 0.2 ng of human genomic DNA) and limited extension time (100 sec at 60oC), HelixAmp™ HyperSense-Taq polymerase reveals superior performance than HelixAmp™ Xtender-Taq polymerase.

Figure 2. The extreme sensitivity of HelixAmp™ HyperSense-Taq polymerase. The sensitivity of HelixAmp™ HyperSense-Taq polymerase was evaluated by the amplification of beta-globin gene with 1.25 units of Xtender-Taq polymerase or HyperSense-Taq polymerase with various concentrations of template. Even from less than 5 copies (0.016 ng) of genomic DNA template, HelixAmp™ HyperSense-Taq polymerase amplifies the target successfully. NC : Negative control without template.
Cat.No. Product Size
 HST250 HelixAmp™ HyperSense-Taq polymerase (including 10x Reaction Buffer) 250 units 
 HST250N HelixAmp™ HyperSense-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
250 units 
 HST500 HelixAmp™ HyperSense-Taq polymerase (including 10x Reaction Buffer) 500 units 
 HST500N HelixAmp™ HyperSense-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 HST2500 HelixAmp™ HyperSense-Taq polymerase (including 10x Reaction Buffer, Blue Box) 2,500 units 
 HST2500N HelixAmp™ HyperSense-Taq polymerase
(including 10x Reaction Buffer, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units