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HelixAmp™ Hot-Taq Polymerase (Ver. 2.0) PCR enzymes Selection Guide    
● Automatic hot start PCR
● Extreme Specificity
● Multiplex PCR
● High performance in Real-time PCR

Description
HelixAmp™ Hot-Taq polymerase (Ver. 2.0)is a chemically-modified form of purified Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The attached heat-labile chemical moiety makes Taq DNA polymerase inactive and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and first ramp-up of thermal cycling. During the first denaturation step of PCR the chemical moieties are released from Taq polymerase and the enzyme turns to be active. With the high specificity HelixAmp™ Hot-Taq polymerase (Ver.2.0)shows high performance in the reactions of genotyping (microsatellite or SNP), mutiplex PCR, and realtime PCR. For the maximum performance extremely pure dNTPs and TuneUp™ solutions are also included. TuneUp™ solution helps the DNA polymerase to efficiently amplify the problematic target region of high G+C content or structural problem.

Application
◎ Hot-Start PCR
◎ Real-Time PCR
◎ Genotyping
◎ Multiplex PCR

Contents

◎ HelixAmp™ Hot-Taq DNA polymerase (2.5 units/)
◎ 10X Reaction Buffer
◎ dNTP Mix (each 10 mM)
◎ 5X TuneUp™ solution


Quality Control
◎ Contamination assay for nucleases (exo- and endo-)
◎ Contamination assay for bacterial host DNA
◎ Activity assay, Sensitivity assay
◎ Performance test for multiplex PCR

Storage buffer
50% Glycerol, 20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Tween 20, 0.5% Nonidet P-40, 1mM PMSF

Data
 

Figure 1. The high specificity of HelixAmp™ Hot-Taq polymerase. The problematic targets of human genomic DNA are successfully amplified with HelixAmp™ Hot-Taq polymerase. The specific amplifications of COMT gene encoding cathecol-O-methyl transferase (COMT) and APOE gene from human genomic DNA template are shown. Manual hot-start was performed as adding Taq DNA polymerase to reaction mixture at 85. M: HelixRuler™ 1 kb(+) DNA ladder.

 

Figure 2. HelixAmp™ Hot-Taq polymerse is highly specific. 100 ng human genomic DNA was used as template. The specificity of HelixAmp™ Hot-Taq polymerase is shown by the amplification of COMT gene encoding cathecol-O-methyl transferase (COMT) compared with Taq polymerase and an antibody-type hot start Taq polymerase.


 

Figure 3. HelixAmp™ Hot-Taq polymerse possesses the high activity. The enzyme activity of HelixAmp™ Hot-Taq polymerase was evaluated by the amplification of COMT, APOE, or SBMA gene from 100 ng human genomic DNA templates with various concentrations of enzyme. The activity comparison was performed with other competitive product of chemically modified hot-start Taq DNA polymerase.
Cat.No. Product Size
 HT250 HelixAmp™ Hot-Taq polymerase (Ver. 2.0) (including 10x Reaction Buffer) 250 units 
 HT500 HelixAmp™ Hot-Taq polymerase (Ver. 2.0) (including 10x Reaction Buffer) 500 units 
 HT2500 HelixAmp™ Hot-Taq polymerase (Ver. 2.0) (including 10x Reaction Buffer, Blue Box) 2,500 units 
 HT250N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
250 units 
 HT500N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Reaction Buffer, TuneUp™ solution, dNTP Mix)
500 units 
 HT2500N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Reaction Buffer, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units 
 HTBF250 HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂)
250 units 
 HTBF500 HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂)
500 units 
 HTBF2500 HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂,Blue Box)
2,500 units 
 HTBF250N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂, TuneUp™ solution, dNTP Mix)
250 units 
 HTBF500N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂, TuneUp™ solution, dNTP Mix)
500 units 
 HTBF2500N HelixAmp™ Hot-Taq polymerase (Ver. 2.0)
(10x Mg-free buffer, 25mM MgCl₂, TuneUp™ solution, dNTP Mix, Blue Box)
2,500 units